tryple tm tm select enzyme (1x), phenol red  (Thermo Fisher)

Journal: Sensors (Basel, Switzerland)

Article Title: Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications

doi: 10.3390/s17071603

Figure Lengend Snippet: Culture of MDCK-cells (scale bar is 100 µ m ): ( a ) overview about the experimental procedure (not to scale); ( b ) t = 0 h: seeding of the cells inside the chip; ( c ) t = 4 h: cells adhere on the bottom plate; ( d ) t = 24 h: cell growth inside the chamber; ( e ) t = 24 h (culture) + 12 min incubation with TrypLE: first cells detach from the plate; ( f ) t = 24 h + 30 min incubation with TrypLE: Detaching of almost all cells; ( g ) t = 24 . 5 h + 45 min : A few cells remain inside the chip after the splitting; ( h ) t = 24 . 5 h + 24 h : on-chip growth of the cells; ( i ) t = 24 . 5 h + 45 min : the majority of the cells is removed from the chip after splitting and added in a 24-well plate; ( j ) t = 24 . 5 h + 24 h : off-chip growth of the cells.

Article Snippet: The detaching of the MDCK-cells is done with TrypLE TM Select Enzym (1x) purchased from ThermoFisher (Waltham, Massachusetts, USA).

Techniques: Incubation